Phylogenetic and stress-responsive expression analysis of 20 WRKY genes in Populus simonii × Populus nigra.
Identifieur interne : 001B99 ( Main/Exploration ); précédent : 001B98; suivant : 001C00Phylogenetic and stress-responsive expression analysis of 20 WRKY genes in Populus simonii × Populus nigra.
Auteurs : Hui Zhao [République populaire de Chine] ; Sui Wang [République populaire de Chine] ; Su Chen [République populaire de Chine] ; Jing Jiang [République populaire de Chine] ; Guifeng Liu [République populaire de Chine]Source :
- Gene [ 1879-0038 ] ; 2015.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Facteurs de transcription (génétique), Phylogenèse (MeSH), Populus (classification), Populus (génétique), Protéines végétales (génétique), Régulation de l'expression des gènes végétaux (MeSH), Résistance à la maladie (MeSH), Stress physiologique (MeSH), Transcriptome (MeSH), Évolution moléculaire (MeSH).
- MESH :
English descriptors
- KwdEn :
- Disease Resistance (MeSH), Evolution, Molecular (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (MeSH), Phylogeny (MeSH), Plant Proteins (genetics), Populus (classification), Populus (genetics), Stress, Physiological (MeSH), Transcription Factors (genetics), Transcriptome (MeSH).
- MESH :
- chemical , genetics : Plant Proteins, Transcription Factors.
- classification : Populus.
- genetics : Populus.
- Disease Resistance, Evolution, Molecular, Gene Expression Profiling, Gene Expression Regulation, Plant, Phylogeny, Stress, Physiological, Transcriptome.
Abstract
WRKY transcription factors play important roles in regulating biotic and abiotic stress responses in plants. Although a plethora of studies have revealed the functions and mechanisms of some WRKYs in various plants, the studies of WRKYs in woody plants especially tree species under different abiotic and biotic stress conditions are still not well characterized. In this study, we selected 20 Populus simonii×Populus nigra WRKY genes based on our previous transcriptome study, and characterized these genes by phylogenetic analysis to investigate their evolutionary relations, then studied their expression patterns under NaCl, NaHCO3, PEG6000, CdCl2 and Alternaria alternata (Fr.) Keissl treatments that mimic the salt, alkalinity, drought, heavy metal and fungal infection conditions. The phylogenetic analysis showed that these 20 genes can be divided into five clades (Groups I, IIa, IIb, IIc and III) and all of their WRKY domains are conserved except for an N-terminal single amino acid mutation in PsnWRKY8. Before conducting quantitative real time PCR calculation, we evaluated five candidate reference genes under different stress treatments, and chose At4g33380-like as the reference gene for salt stress, Actin for alkalinity stress, UBQ for drought stress, TUA for heavy metal stress, and 18S rRNA for pathogen infection stress. The final qRT-PCR analysis indicated that 20/20, 20/20, and 15/20 PsnWRKYs were downregulated under salt, alkali and drought stresses, and 14/20 and 19/20 PsnWRKYs were upregulated under heavy metal and pathogen stresses. Members from the same clade tended to present similar expression patterns. In addition, we observed noticeable changes in the expression of PsnWRKY11 (increased by 41 times) and PsnWRKY20 (increased by 141 times) under pathogen infection condition, implying that these two genes are potentially important for the disease resistance of P. simonii × P. nigra.
DOI: 10.1016/j.gene.2015.04.002
PubMed: 25843624
Affiliations:
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Electronic address: liuguifeng.lgf@gmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author></analytic><series><title level="j">Gene</title><idno type="eISSN">1879-0038</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Disease Resistance (MeSH)</term><term>Evolution, Molecular (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (genetics)</term><term>Populus (classification)</term><term>Populus (genetics)</term><term>Stress, Physiological (MeSH)</term><term>Transcription Factors (genetics)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Facteurs de transcription (génétique)</term><term>Phylogenèse (MeSH)</term><term>Populus (classification)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Résistance à la maladie (MeSH)</term><term>Stress physiologique (MeSH)</term><term>Transcriptome (MeSH)</term><term>Évolution moléculaire (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Disease Resistance</term><term>Evolution, Molecular</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Phylogeny</term><term>Stress, Physiological</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Phylogenèse</term><term>Régulation de l'expression des gènes végétaux</term><term>Résistance à la maladie</term><term>Stress physiologique</term><term>Transcriptome</term><term>Évolution moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">WRKY transcription factors play important roles in regulating biotic and abiotic stress responses in plants. Although a plethora of studies have revealed the functions and mechanisms of some WRKYs in various plants, the studies of WRKYs in woody plants especially tree species under different abiotic and biotic stress conditions are still not well characterized. In this study, we selected 20 Populus simonii×Populus nigra WRKY genes based on our previous transcriptome study, and characterized these genes by phylogenetic analysis to investigate their evolutionary relations, then studied their expression patterns under NaCl, NaHCO3, PEG6000, CdCl2 and Alternaria alternata (Fr.) Keissl treatments that mimic the salt, alkalinity, drought, heavy metal and fungal infection conditions. The phylogenetic analysis showed that these 20 genes can be divided into five clades (Groups I, IIa, IIb, IIc and III) and all of their WRKY domains are conserved except for an N-terminal single amino acid mutation in PsnWRKY8. Before conducting quantitative real time PCR calculation, we evaluated five candidate reference genes under different stress treatments, and chose At4g33380-like as the reference gene for salt stress, Actin for alkalinity stress, UBQ for drought stress, TUA for heavy metal stress, and 18S rRNA for pathogen infection stress. The final qRT-PCR analysis indicated that 20/20, 20/20, and 15/20 PsnWRKYs were downregulated under salt, alkali and drought stresses, and 14/20 and 19/20 PsnWRKYs were upregulated under heavy metal and pathogen stresses. Members from the same clade tended to present similar expression patterns. In addition, we observed noticeable changes in the expression of PsnWRKY11 (increased by 41 times) and PsnWRKY20 (increased by 141 times) under pathogen infection condition, implying that these two genes are potentially important for the disease resistance of P. simonii × P. nigra.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25843624</PMID><DateCompleted><Year>2015</Year><Month>07</Month><Day>02</Day></DateCompleted><DateRevised><Year>2015</Year><Month>05</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0038</ISSN><JournalIssue CitedMedium="Internet"><Volume>565</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Jul</Month><Day>01</Day></PubDate></JournalIssue><Title>Gene</Title><ISOAbbreviation>Gene</ISOAbbreviation></Journal><ArticleTitle>Phylogenetic and stress-responsive expression analysis of 20 WRKY genes in Populus simonii × Populus nigra.</ArticleTitle><Pagination><MedlinePgn>130-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.gene.2015.04.002</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0378-1119(15)00379-0</ELocationID><Abstract><AbstractText>WRKY transcription factors play important roles in regulating biotic and abiotic stress responses in plants. Although a plethora of studies have revealed the functions and mechanisms of some WRKYs in various plants, the studies of WRKYs in woody plants especially tree species under different abiotic and biotic stress conditions are still not well characterized. In this study, we selected 20 Populus simonii×Populus nigra WRKY genes based on our previous transcriptome study, and characterized these genes by phylogenetic analysis to investigate their evolutionary relations, then studied their expression patterns under NaCl, NaHCO3, PEG6000, CdCl2 and Alternaria alternata (Fr.) Keissl treatments that mimic the salt, alkalinity, drought, heavy metal and fungal infection conditions. The phylogenetic analysis showed that these 20 genes can be divided into five clades (Groups I, IIa, IIb, IIc and III) and all of their WRKY domains are conserved except for an N-terminal single amino acid mutation in PsnWRKY8. Before conducting quantitative real time PCR calculation, we evaluated five candidate reference genes under different stress treatments, and chose At4g33380-like as the reference gene for salt stress, Actin for alkalinity stress, UBQ for drought stress, TUA for heavy metal stress, and 18S rRNA for pathogen infection stress. The final qRT-PCR analysis indicated that 20/20, 20/20, and 15/20 PsnWRKYs were downregulated under salt, alkali and drought stresses, and 14/20 and 19/20 PsnWRKYs were upregulated under heavy metal and pathogen stresses. Members from the same clade tended to present similar expression patterns. In addition, we observed noticeable changes in the expression of PsnWRKY11 (increased by 41 times) and PsnWRKY20 (increased by 141 times) under pathogen infection condition, implying that these two genes are potentially important for the disease resistance of P. simonii × P. nigra.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Hui</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Sui</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Su</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Guifeng</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China. Electronic address: liuguifeng.lgf@gmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Gene</MedlineTA><NlmUniqueID>7706761</NlmUniqueID><ISSNLinking>0378-1119</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019143" MajorTopicYN="N">Evolution, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="Y">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="N">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Expression pattern</Keyword><Keyword MajorTopicYN="N">Phylogenetic</Keyword><Keyword MajorTopicYN="N">Populus simonii×Populus nigra</Keyword><Keyword MajorTopicYN="N">Stress</Keyword><Keyword MajorTopicYN="N">WRKY gene</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>04</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>01</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>7</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25843624</ArticleId><ArticleId IdType="pii">S0378-1119(15)00379-0</ArticleId><ArticleId IdType="doi">10.1016/j.gene.2015.04.002</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Hui" sort="Zhao, Hui" uniqKey="Zhao H" first="Hui" last="Zhao">Hui Zhao</name></noRegion><name sortKey="Chen, Su" sort="Chen, Su" uniqKey="Chen S" first="Su" last="Chen">Su Chen</name><name sortKey="Jiang, Jing" sort="Jiang, Jing" uniqKey="Jiang J" first="Jing" last="Jiang">Jing Jiang</name><name sortKey="Liu, Guifeng" sort="Liu, Guifeng" uniqKey="Liu G" first="Guifeng" last="Liu">Guifeng Liu</name><name sortKey="Wang, Sui" sort="Wang, Sui" uniqKey="Wang S" first="Sui" last="Wang">Sui Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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